Effect of the Neck Linker on Processive Stepping of Kinesin Motor

Kinesin motor protein, which is composed of two catalytic domains connected together by a long coiled-coil stalk via flexible neck linkers (NLs), can step processively on microtubule towards the plus end hydrolyzing adenosine triphosphate (ATP) molecules. To understand what role that NL plays in processive stepping, dynamics kinesin are studied theoretically here considering mutation or deletion an N-terminal cover strand contributes to docking kinesin-1, extension swapping kinesin-2 with joining and Ncd moves minus MT domain replacement kinesin-1 Ncd, so on. The theoretical results give consistent quantitative explanation various available experimental about effects these mutations and, moreover, provide predicted results. Additionally, motility kinesin-6 MKLP2 without also explained. data effect bi-directional kinesin-5 Cin8 studies critically implicative mechanism stepping motor.